tissue-resident macrophages

 

 

Ghosn EE et al. Two physically, functionally, and developmentally distinct peritoneal macrophage subsets. PNAS 2010

 

The myeloid lineage comprises several functionally and developmentally distinct subsets, including circulating monocytes, and tissue-resident macrophages. Several studies have shown that tissue-resident macrophages, but not circulating monocytes, emerge from the yolk sac during embryogenesis independent of the LT-HSC. Brain and skin resident macrophages (i.e., microglia and Langerhans cells, respectively) emerge at around embryonic day 8 (E8) in a region of the yolk sac known as the blood island, even before the development of the first definitive LT-HSC. The yolk sac-derived (HSC-independent) macrophages then migrate and take long-term residence in the brain and skin of the developing embryo. 

Single-cell HTP qPCR (96 cells x 96 genes) reveals distinct gene expression profile for SPM and LPM

Recently, we have identified and characterized two functionally distinct subsets of tissue-resident peritoneal macrophages named small (SPM) and large (LPM) peritoneal macrophages. Currently, we are studying their origin and functional relationship with other tissue-resident macrophages, such as brain microglia, liver Kupffer, and skin Langerhans cells.

 

SPM and LPM macrophage subsets are phenotypically and morphologically distinguished by both flow cytometry and microscopy. Ghosn EE et al. PNAS 2010

 
 
 
 

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