publications

 
 

research articles and reviews

  1. Transient anti-interferon autoantibodies in the airways are associated with recovery from COVID-19. Babcock BR, Kosters A, Eddins DJ, Donaire MSB, Sarvadhavabhatla S, Pae V, Beltran F, Murray VW, Gill G, Xie G, Dobosh BS, Giacalone VD, Tirouvanziam RM, Ramonell RP, Jenks SA, Sanz I, Lee FEH, Roan NR, Lee SA, Ghosn EE*. Sci. Transl. Med. 2024 | cover art | Emory News | Axios | iNews

  2. Disease-associated B cells and immune endotypes shape adaptive immune responses to SARS-CoV-2 mRNA vaccination in human SLE. Faliti  CA, Van TTP, Anam FA, Cheedarla N, Williams ME, Mishra AK, Usman SY, Woodruff MC, Kraker G, Runnstrom MC, Kyu S, Sanz D, Ahmed H, Ghimire M, Morrison-Porter A, Quehl H, Haddad NS, Chen W, Cheedarla S, Neish AS, Roback JD, Antia R, Hom J, Tipton CM, Lindner JM, Ghosn EE, Khurana S, Scharer CD, Khosroshahi A, Lee FEH, Sanz I. Nat. Immunol. 2024

  3. Lifting the curse from high dimensional data: Automated projection pursuit clustering for the variety of biological data modalities. Simpson C, Tabatsky E, Rahil Z, Eddins DJ, Tkachev S, Georgescauld F, Papalegis D, Culka M, Levy T, Gregoretti I, Chernyshev A, Koeppen H, Walther G, Ghosn EE, Orlova D. bioRxiv 2024

  4. Understanding heterogeneity of human bone marrow plasma cell maturation and survival pathways by single-cell analyses. Duan M, Nguyen DC, Joyner CJ, Saney CL, Tipton CM, Andrews J, Lonial S, Kim C, Hentenaar I, Kosters A, Ghosn EE, Jackson A, Knechtle S, Maruthamuthu S, Chandran S, Martin T, Rajalingam R, Vincenti F, Breeden C, Sanz I, Gibson G, Lee FEH. Cell Rep. 2023

  5. Stromal STAT5-mediated trophic activity regulates hematopoietic niche factors. Wang Z, Emmel G, Lim HS, Zhu W, Kosters A, Ghosn EE, Qiu P, Bunting, KD. Stem Cells 2023

  6. Monocyte depletion enhances neutrophil influx and proneural to mesenchymal transition in glioblastoma. Chen Z, Soni N, Pinero G, Giotti B, Eddins DJ, Lindblad KE, Ross JL, Vallcorba MP, Joshi T, Angione A, Thomason W, Keane A, Tsankova NM, Gutmann DH, Lira SA, Lujambio A, Ghosn EE, Tsankov AM, Hambardzumyan D. Nat. Commun. 2023

  7. Transcriptional reprogramming of infiltrating neutrophils drives lung pathology in severe COVID-19 despite low viral load. Eddins DJ, Yang J, Kosters A, Giacalone V, Pechuan X, Chandler JD, Eum J, Babcock BR, Dobosh BS, Hernández MR, Abdulkhader F, Collins GL, Orlova DY, Ramonell RP, Sanz I, Moussion C, Lee FE, Tirouvanziam RM, Ghosn EE*. Blood Adv. 2023 | featured on Lab Land Blog

  8. Profiling the peripheral immune response to ex vivo TNF stimulation in untreated juvenile idiopathic arthritis using single-cell RNA sequencing. Imbach K, Treadway N, Prahalad V, Kosters A, Arafat D, Duan M, Gergely T, Ponder L, Chandrakasan S, Ghosn EE, Prahalad S, Gibson G. Pediatric Rheumatology 2023

  9. Assessing cellular and transcriptional diversity of ileal mucosa among treatment-naïve and treated Crohn’s disease. Maddipatla SC, Kolachala VL, Venkateswaran S, Dodd AF, Pelia RS, Geem D, Yin H, Sun Y, Xu C, Mo A, Kosters A, Yang J, Matthews JD, Ghosn EE, Kugathasan S, Qiu P. Inflamm. Bowel Dis. 2023

  10. Comprehensive multi-omics single-cell data integration reveals greater heterogeneity in the human immune system. Xu C, Yang J, Kosters A, Babcock BR, Qiu P*, Ghosn EE*. iScience 2022

  11. Mast cell repopulating ability is lost during the transition from pre-HSC to FL HSC. Yoshimoto M*, Kosters A, Cornelius S, Valiente N, Cheng H, Latorre A, Nishida C, Ghosn EE*, Kobayashi M*. Front. Immunol. 2022

  12. Baricitinib attenuates the proinflammatory phase of COVID-19 driven by lung-infiltrating monocytes. Dobosh B, Zandi K, Giraldo DM, Goh SL, Musall K, Aldeco M, LeCher J, Giacalone VD, Yang J, Eddins DJ, Bhasin M, Ghosn EE, Sukhatme V, Schinazi RF, Tirouvanziam R. Cell Rep. 2022 | featured on medical.net & Emory News Center

  13. Regulation of the BCR signalosome by the class II peptide editor, H2-M, affects the development and repertoire of innate-like B cells. Ghosh D*, Pham TD, Nanaware PP, Sengupta D, Adler LN, Li CG, He X, O'Mara ME, Kantor AB, Nguyen KD, Yang Y, Eisenlohr LC, Jensen PE, Herzenberg LA, Stern LJ, Boyd SD, Ghosn EE*, Mellins ED*. Cell Rep. 2022

  14. Inactivation of SARS-CoV-2 and COVID-19 patient samples for contemporary immunology and metabolomics studies. Eddins DJ, Bassit L, Chandler JD, Haddad NS, Musall KL, Yang J, Kosters A, Dobosh BS, Hernández MR, Ramonell RP, Tirouvanziam RM, Lee FE, Zandi K, Schinazi RF, Ghosn EE*. ImmunoHorizons 2022

  15. Data matrix normalization and merging strategies minimize batch-specific systemic variation in scRNA-seq data. Babcock BR, Kosters A, Yang J, White ML, Ghosn EE*. bioRxiv 2021

  16. Ultraselective carbon nanotubes for photoacoustic imaging of inflamed atherosclerotic plaques. Gifani M, Eddins DJ, Kosuge H, Zhang Y, Paluri SLA, Larson T, Leeper N, Herzenberg LA, Gambhir SS, McConnell MV, Ghosn EE*, Smith BR*. Adv. Funct. Mater. 2021 | cover art | featured on Lab Land Blog

  17. Protracted yet coordinated differentiation of long-lived SARS-CoV-2-specific CD8+ T cells during COVID-19 convalescence. Ma T, Ryu H, McGregor M, Babcock B, Neidleman J, Xie G, George AF, Frouard J, Murray V, Gill G, Ghosn EE, Newell E, Lee S, Roan NR. J. Immunol. 2021

  18. Hematopoietic stem cell requirement for macrophage regeneration is tissue-specific. Eddins DJ, Kosters K, Waters J, Sosa J, Phillips M, Yadava K, Herzenberg LA, Kuipers HF, Ghosn EE*. J. Immunol. 2021 | cover art

  19. Distinctive features of SARS-CoV-2-specific T cells predict recovery from severe COVID-19. Neidleman J, Luo X, George AF, McGregor M, Yang J, Yun C, Murray V, Gill G, Greene WC, Vasquez J, Lee S, Ghosn EE*, Lynch K*, Roan NR*. Cell Rep. 2021

  20. Extrafollicular B cell responses correlate with neutralizing antibodies and morbidity in COVID-19. Woodruff MC, Ramonell RP, Nguyen D, Cashman K, Saini AS, Haddad N, Ley AM, Kyu S, Howell JC, Ozturk T, Lee S, Suryadevara N, Case JB, Bugrovsky R, Chen W, Estrada J, Morrison-Porter A, Derrico A, Anam FA, Sharma M, Wu HM, Le SN, Jenks SA, Tipton CM, Staitieh B, Daiss JL, Ghosn EE, Diamond MS, Carnahan RH, Crowe Jr. JE, Hu WT, Lee FE, Sanz I. Nat. Immunol. 2020

  21. SARS-CoV-2-Specific T Cells Exhibit Phenotypic Features of Helper Function, Lack of Terminal Differentiation, and High Proliferation Potential. Neidleman J, Luo X, Frouard J, Xie G, Gurjot G, Stein E, McGregor M, Ma T, George A, Kosters A, Greene W, Vasquez J, Ghosn EE, Lee S, Roan NR. Cell Rep. Med. 2020

  22. Bmi1 Maintains the Self-Renewal Property of Innate-like B Lymphocytes. Kobayashi M, Lin Y, Mishra A, Shelly C, Gao R, Reeh C, Wang P, Xi R, Liu Y, Wenzel P, Ghosn EE, Liu Y, Yoshimoto M. J. Immunol. 2020

  23. HSC-independent hematopoiesis and the origins of innate-like B lymphocytes. Ghosn EE*, Yoshimoto M, Nakauchi H, Weissman I, Herzenberg LA. Development 2019

  24. Automated subset identification and characterization pipeline for multidimensional flow and mass cytometry data clustering and visualization. Meehan S, Kolyagin G, Parks D, Youngyunpipatkul J, Herzenberg L, Walther G, Ghosn EE, Orlova D. Commun. Biol. 2019

  25. On the origins of B-1 B cells. Ghosn EE*, Dorshkind K, Herzenberg LA, Holodick N, Kantor A, Montecino-Rodrigues E, Rothstein T, Silverman GJ, Yang Y, Yoder MC, Yoshimoto M. Science 2019 | eLetter

  26. QFMatch: multidimensional flow and mass cytometry samples alignment. Orlova D, Meehan S, Parks D, Moore W, Meehan C, Zhao Q, Ghosn EE, Herzenberg L, Walther G. Sci. Rep. 2018

  27. Evidence for altered levels of IgD in the nasal airway mucosa of patients with chronic rhinosinusitis. Min J, Raju P, Hulse K, Stevens W, Huang J, Van Roey G, Norton J, Suh L, Carter R, Price C, Weibman A, Rashan A, Ghosn EE, Homma T, Conley D, Welch K, Shintani-Smith S, Peters A, Grammer III L, Harris K, Kato A, Hwang P, Kern R, Herzenberg L, Schleimer R, Nayak J, Tan B. JACI 2017

  28. Fetal hematopoietic stem cell transplantation fails to fully regenerate the B-lymphocyte compartment. Ghosn EE*, Waters, J, Phillips M, Yamamoto R, Long B, Yang Y, Gerstein R, Stoddart C, Nakauchi H, Herzenberg, LA. Stem Cell Rep. 2016 | recommended in F1000 prime

  29. Earth Mover's Distance (EMD): a true metric for comparing biomarker expression levels in cell populations. Orlova D, Zimmerman N, Meehan S, Meehan C, Waters J, Ghosn EE, Filatenkov A, Kolyagin G, Gernez Y, Moss R, Herzenberg LA, Walther G. PLoS One 2016

  30. Characterization of immunoglobulin E plasma cells that are elevated in the upper airway mucosa of nonatopic patients with chronic rhinosinusitis without nasal polyps. Rashan A, Ghosn EE, Peterson A, Yang Y, Phillips M, Sahaf B, Herzenberg LA, Nayak JV. Int. Forum Allergy Rhinol. 2016

  31. Amyloid fibrils activate B-1a lymphocytes to ameliorate inflammatory brain disease. Kurnellas MP, Ghosn EE, Schartner JM, Baker J, Rothbard J, Negrin R, Herzenberg LA, Fathman CG, Steinman L, Rothbard JB. PNAS 2015

  32. Distinct mechanisms define murine B cell lineage immunoglobulin heavy chain (IgH) repertoires. Yang Y, Wang C, Yang Q, Kantor AB, Chu H, Ghosn EE, Qin G, Mazmanian SK, Han J, Herzenberg LA. eLife 2015

  33. Hematopoietic stem cell-independent B-1a lineage. Ghosn EE* & Yang Yang. Ann. N. Y. Acad. Sci. 2015

  34. Selective uptake of single-walled carbon nanotubes by circulating monocytes for enhanced tumour delivery. Smith B, Ghosn EE, Rallapalli H, Prescher J, Herzenberg LA, Gambhir SS. Nat. Nanotechnol. 2014

  35. AutoGate: automating analysis of flow cytometry data. Meehan S, Walther G, Moore W, Orlova D, Meehan C, Parks D, Ghosn EE, Philips M, Mitsunaga E, Waters J, Kantor A, Okamura R, Owumi S, Yang Y, Herzenberg LA and Herzenberg LA. Immunol Res. 2014

  36. Intracellular localization of myeloperoxidase in murine peritoneal B-lymphocytes and macrophages. Araujo T, Okada S, Ghosn EE, Taniwaki NN, Rodrigues MR, Almeida SR, Mortara RA, Russo M, Campa A, Albuquerque RC. Cell Immunol. 2013

  37. Reversal of Paralysis and Reduced Inflammation from Peripheral Administration of β-Amyloid in TH1 and TH17 Versions of Experimental Autoimmune Encephalomyelitis. Grant J, Ghosn EE, Axtell RC, Herges K, Kuipers HF, Woodling NS, Andreasson K, Herzenberg LA, Herzenberg LA, Steinman L. Sci. Transl. Med. 2012 | cover art

  38. Distinct B-cell lineage commitment distinguishes adult bone marrow hematopoietic stem cells. Ghosn EE*, Yamamoto R, Hamanaka S, Yang Y, Herzenberg LA, Nakauchi H, Herzenberg LA. PNAS 2012 | highlighted in In This Issue

  39. Development of B cells and erythrocytes is specifically impaired by the drug celastrol in mice. Kusy S, Ghosn EE, Herzenberg LA, Contag CH. PLoS One 2012

  40. Antigen-specific memory in B-1a and its relationship to natural immunity. Yang Y, Ghosn EE, Cole LE, Obukhanych TV, Sadate-Ngatchou P, Vogel SN, Herzenberg LA, Herzenberg LA. PNAS 2012

  41. Antigen-specific antibody responses in B-1 and their relationship to natural immunity. Yang Y, Ghosn EE, Cole LE, Obukhanych TV, Sadate-Ngatchou P, Vogel SN, Herzenberg LA, Herzenberg LA. PNAS 2012

  42. Cellular renewal and improvement of local cell effector activity in peritoneal cavity in response to infectious stimuli. Cassado A, de Albuquerque J, Sardinha L, Buzzo CL, Faustino L, Nascimento R, Ghosn EE, Lima MR, Alvarez JM, Bortoluci KR. PLoS One 2011

  43. Distinct progenitors for B-1 and B-2 cells are present in adult mouse spleen. Ghosn EE*, Sadate-Ngatchou P, Yang Y, Herzenberg LA, Herzenberg LA. PNAS 2011

  44. Basophil CD203c levels are increased at baseline and can be used to monitor omalizumab treatment in subjects with nut allergy. Gernez Y, Tirouvanziam R, Yu G, Ghosn EE, Reshawmala N, Nguyen T, Tsai M, Galli SJ, Herzenberg LA, Nadeau KC. Int. Arch. Allergy Immunol. 2011

  45. Two physically, functionally and developmentally distinct peritoneal macrophage subsets. Ghosn EE*, Cassado A, Govoni G, Fukuhara T, Yang Y, Monack D, Bortoluci K, Almeida SR, Herzenberg LA. PNAS 2010

  46. Monocyte-derived dendritic cells from patients with severe forms of chromoblastomycosis induce CD4+ T cell activation in vitro. Sousa MG, Ghosn EE, Nascimento RC, Bomfim GF, Noal V, Santiago K, Silva Azevedo CM, Marques SG, Gonçalves AG, Lima Santos DW, Criado PR, Costa Martins JE, Almeida SR. Clin. Exp. Immunol. 2009

  47. CD11b expression distinguishes sequential stages of peritoneal B-1 development. Ghosn EE, Yang Y, Tung J, Herzenberg LA. PNAS 2008

  48. Fonsecaea pedrosoi infection induces differential modulation of co-stimulatory molecules and cytokines in monocytes from patients with severe and mild forms of chromoblastomycosis. Sousa MG, Azevedo C, Nascimento R, Ghosn EE, Santiago KL, Noal V, Bomfim GF, Marques SG, Gonçalves AG, Wagner de Castro D, Almeida SR. J. Leukoc. Biol. 2008

  49. Division and differentiation of natural antibody-producing cells in mouse spleen. Yang Y, Tung J, Ghosn EE, Herzenberg LA. PNAS 2007

  50. Absence of CD4+ T cells impairs host defense of mice infected with Fonsecaea pedrosoi. Sousa MG, Ghosn EE, Almeida SR. Scand. J. Immunol. 2006

  51. Phagocytosis, production of nitric oxide and pro-inflammatory cytokines by macrophages in the presence of dematiaceous fungi that cause chromoblastomycosis. Hayakawa M, Ghosn EE, Sousa MG, Ferreira KS, Almeida SR. Scand. J. Immunol. 2006

  52. Nitric oxide-dependent killing of Cryptococcus neoformans by B-1 derived mononuclear phagocyte. Ghosn EE, Russo M, Almeida SR. J. Leukoc. Biol. 2006

 protocols

  1. Human intestinal cell dissociation suitable for multi-omics single-cell assays. Kosters A, Yang J, Dodd A, White ML, Gibson G, Kugathasan S, Qiu P, Ghosn E. protocols.io 2021

  2. Murine intestinal cell dissociation suitable for multi-omics single-cell assays. White ML, Kosters A, Cervantes-Barragan L, Ghosn E. protocols.io 2021

books & book chapters

  1. B-1 cell development and Function. Editors: Rothstein T, Herzenberg LA, Holodick N, Ghosn EE. Ann NY Acad Sci., Volume 1362, Issue 1, 2015

  2. Autogate, an automated approach to flow data analysis | pages 260-265. Herzenberg LA, Meehan S, Walther G, Parks D, Moore W, Meehan C, Philips M, Ghosn EE, Herzenberg LA. In: Jikiden Flow Cytometry, First Edition, Yodosha Co., Ltd., Japan, 2014

  3. Severe Mycosis (Micoses Profundas) | pages 94-104. Ghosn EE. In: Micologia, First Edition, Guanabara Koogan S.A., Brazil, 2008

  4. Opportunistic Mycosis (Micoses Oportunistas) | pages 105-122. Ghosn EE. In: Micologia, First Edition, Guanabara Koogan S.A., Brazil, 2008